2020年

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• Masuhara K., Akatsuka H., Tokusanai M., Li C., Iida Y., Okada Y., Suzuki T., Ohtsuka M., Inoue I., Kimura M., Hosokawa H., Hozumi K. and Sato T. AMBRA1 controls antigen-driven activation and proliferation of naïve T cells. Int Immunol. 2020 (in press)

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• Kotaki R., Kawashima M., Yamaguchi A., Suzuki N., Koyama-Nasu R., Ogiya D., Okuyama K., Yamamoto Y., Takamatsu M., Kurosaki N., Ando K., Murata A., Ohtsuka M., Nakagawa S., Katagiri K. and Kotani A. Overexpression of miR-669m inhibits erythroblast differentiation. Sci Rep. Aug 11;10(1):13554. (2020)

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• Oka A., Takagi A., Komiyama E., Yoshihara N., Mano S., Hosomichi K., Suzuki S., Haida Y., Motosugi N., Hatanaka T., Kimura M., Ueda MT., Nakagawa S., Miura H., Ohtsuka M., Tanaka M., Komiyama T., Otomo A., Hadano S., Mabuchi T., Beck S., Inoko H. and Ikeda S. Alopecia areata susceptibility variant in MHC region impacts expressions of genes contributing to hair keratinization and is involved in hair loss. EBioMedicine. Jul;57:102810. (2020)

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• 大塚正人：i-GONAD法によるゲノム編集動物の作製.医学のあゆみ. 273, 780-784. (2020)

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• Hayashi H., Wang T., Tanaka M., Ogiwara S., Okada C., Ito M., Fukunishi N., Iida Y., Nakamura A., Sasaki A., Amano S., Yoshida K., Otomo A., Ohtsuka M. and Hadano S. Monitoring the autophagy-endolysosomal system using monomeric Keima-fused MAP1LC3B. PLoS ONE 15(6): e0234180. (2020)

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• Tamura S., Yasuoka Y., Miura H., Takahashi G., Sato M. and Ohtsuka M. Thy1 promoter activity in the Rosa26 locus in mice: lessons from Dre-rox conditional expression system. Exp. Anim. in press.

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• Kobayashi Y., Aoshima T., Ito R., Shinmura R., Ohtsuka M., Akasaka E., Sato M. and Takabayashi S. Modification of i-GONAD Suitable for Production of Genome-Edited C57BL/6 Inbred Mouse Strain. Cells 9, 957. (2020)

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• Sato M., Miyagasako R., Takabayashi S., Ohtsuka M., Hatada I. and Horii T. Sequential i-GONAD: An Improved In Vivo Technique for CRISPR/Cas9-Based Genetic Manipulations in Mice. Cells 9, 546. (2020)

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• 大塚正人、佐藤正宏：体外での胚操作不要のゲノム編集動物作製法 i-GONAD. 実験医学. 38, 479-487. (2020)

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• Kamimura K., Yokoo T., Abe H., Sakai N., Nagoya T., Kobayashi Y., Ohtsuka M., Miura H., Sakamaki A., Kamimura H., Miyamura N., Nishina H. and Terai S. Effect of Diphtheria Toxin-Based Gene Therapy for Hepatocellular Carcinoma. Cancers 12, 472. (2020)

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• Hirose M., Honda A., Fulka H., Tamura-Nakano M., Matoba S., Tomishima T., Mochida K., Hasegawa A., Nagashima K., Inoue K., Ohtsuka M., Baba T., Yanagimachi R. and Ogura A., Acrosin is essential for sperm penetration through the zona pellucida in hamsters, PNAS 117 (5) 2513-2518 (2020)

2019年

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• Iijima Y., Tanaka M., Suzuki S., Hauser D., Tanaka M., Okada C., Ito M., Ayukawa N., Sato Y., Ohtsuka M., Scheiffele P. and Iijima T. SAM68-Specific Splicing Is Required for Proper Selection of Alternative 3' UTR Isoforms in the Nervous System. iScience. 22: 318-335. (2019)

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• Gurumurthy CB., O'Brien AR., Quadros RM., Adams J. Jr, Alcaide P., Ayabe S., Ballard J., Batra SK., Beauchamp MC., Becker KA., Bernas G., Brough D., Carrillo-Salinas F., Chan W., Chen H., Dawson R., DeMambro V., D'Hont J., Dibb KM., Eudy JD., Gan L., Gao J., Gonzales A., Guntur AR., Guo H., Harms DW., Harrington A., Hentges KE., Humphreys N., Imai S., Ishii H., Iwama M., Jonasch E., Karolak M., Keavney B., Khin NC., Konno M., Kotani Y., Kunihiro Y., Lakshmanan I., Larochelle C., Lawrence CB., Li L., Lindner V., Liu XD., Lopez-Castejon G., Loudon A., Lowe J., Jerome-Majewska LA., Matsusaka T., Miura H., Miyasaka Y., Morpurgo B., Motyl K., Nabeshima YI., Nakade K., Nakashiba T., Nakashima K., Obata Y., Ogiwara S., Ouellet M., Oxburgh L., Piltz S., Pinz I., Ponnusamy MP., Ray D., Redder RJ., Rosen CJ., Ross N., Ruhe MT., Ryzhova L., Salvador AM., Alam SS., Sedlacek R., Sharma K., Smith C., Staes K., Starrs L., Sugiyama F., Takahashi S., Tanaka T., Trafford AW., Uno Y., Vanhoutte L., Vanrockeghem F., Willis BJ., Wright CS., Yamauchi Y., Yi X., Yoshimi K., Zhang X., Zhang Y., Ohtsuka M., Das S., Garry DJ., Hochepied T., Thomas P., Parker-Thornburg J., Adamson AD., Yoshiki A., Schmouth JF., Golovko A., Thompson WR., Lloyd KCK., Wood JA., Cowan M., Mashimo T., Mizuno S., Zhu H., Kasparek P., Liaw L., Miano JM. and Burgio G. Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation. Genome Biol. 20: 171. (2019)

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• Gurumurthy CB., Sato M., Nakamura A., Inui M., Kawano N., Islam MA., Ogiwara S., Takabayashi S., Matsuyama M., Nakagawa S., Miura H. and Ohtsuka M. Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD. Nat Protoc. 14: 2452-2482. (2019)

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• Ohtsuka M. and Sato M. i-GONAD: A method for generating genome-edited animals without ex vivo handling of embryos. Dev Growth Differ. 61: 306-315 (2019)

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• 大塚正人：GONAD法：胚の体外操作を要しない新規ゲノム編集動物作製法．バイオサイエンスとインダストリー. 77, 226-229. (2019)

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• Saiga R., Hoshino M., Takeuchi A., Uesugi K., Naitou K., Kamijo A., Kawabe N., Ohtsuka M., Takizawa S. and Mizutani R. Synchrotron radiation microtomography of brain hemisphere and spinal cord of a mouse model of multiple sclerosis revealed a correlation between capillary dilation and clinical score. J Comp Neurol. 527: 2091-2100. (2019)

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• Sakurai T., Kamiyoshi A., Ohtsuka M., Gurumurthy CB., Sato M. and Shindo T. Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line. Methods Mol Biol. 1874: 257-271 (2019)

2018年

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• Nakamura S., Ishihara M., Watanabe S., Ando N., Ohtsuka M. and Sato M. Intravenous Delivery of piggyBac Transposons as a Useful Tool for Liver-Specific Gene-Switching. Int J Mol Sci. 19: E3452 (2018)

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• Takabayashi S., Aoshima T., Kabashima K., Aoto K., Ohtsuka M. and Sato M. i-GONAD (improved genome-editing via oviductal nucleic acids delivery), a convenient in vivo tool to produce genome-edited rats. Sci Rep. 8: 12059 (2018)

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• Sato M., Ohtsuka M., Nakamura S., Sakurai T., Watanabe S. and Gurumurthy CB. In vivo genome editing targeted towards the female reproductive system. Arch Pharm Res. 41: 898-910 (2018)

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• Kobayashi T., Namba M., Koyano T., Fukushima M., Sato M., Ohtsuka M. and Matsuyama M. Successful production of genome-edited rats by the rGONAD method. BMC Biotechnol.18:19 (2018)

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• Ibi M., Horie S., Kyakumoto S., Chosa N., Yoshida M., Kamo M., Ohtsuka M. and Ishisaki A. Cell-cell interactions between monocytes/macrophages and synoviocyte-like cells promote inflammatory cell infiltration mediated by augmentation of MCP-1 production in temporomandibular joint. Biosci Rep. 38 (2018)

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• Ohtsuka M., Sato M., Miura H., Takabayashi S., Matsuyama M., Koyano T., Arifin N., Nakamura S., Wada K. and Gurumurthy CB. i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases. Genome Biol. 19: 25 (2018)

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• Miura H.†, Quadros RM.†, Gurumurthy CB.* and Ohtsuka M.* Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors. Nat Protoc., 13: 195-215 (2018) †equal contribution, *correspondence

• Sato M., Maeda K., Koriyama M., Inada E., Saitoh I., Ohtsuka M., Nakamura S., Sakurai T., Watanabe S. and Miyoshi K. Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes. Theriogenology, 108: 29-38. (2018)

2017年

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• Sato M., Miyoshi K., Nakamura S., Ohtsuka M., Nakamura S., Sakurai T., Watanabe S. and Kawaguchi H. and Tanimoto A. Efficient generation of somatic cell nuclear transfer-competent porcine cells with mutated alleles at multiple target loci by using CRISPR/Cas9 combined with targeted toxin-based selection system. Int J Mol Sci., 18: 2610 (2017)

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• 大塚正人、三浦浩美：PITT法によるターゲットトランスジェニックマウス作製．動物／疾患モデルの作製技術・病態解析・評価手法　技術情報協会（2017）

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• Akatsuka H., Kuga S., Masuhara K., Davaadorj O., Okada C., Iida Y., Okada Y., Fukunishi N., Suzuki T., Hosomichi K., Ohtsuka M., Tanaka M., Inoue I., Kimura M. and Sato T. AMBRA1 is involved in T cell receptor-mediated metabolic reprogramming through an ATG7-independent pathway. Biochem Biophys Res Commun., 491: 1098-1104 (2017)

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• Takizawa N.*, Okubo N.*, Kamo M., Chosa N., Mikami T., Suzuki K., Yokota S., Ibi M., Ohtsuka M., Taira M., Yaegashi T., Ishisaki A., Kyakumoto S. Bone marrow-derived mesenchymal stem cells propagate immunosuppressive/anti-inflammatory macrophages in cell-to-cell contact-independent and -dependent manners under hypoxic culture. Experimental Cell Research, 358: 411-420. *co-first authors.

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• Jacobi AM., Rettig GR., Turk R., Collingwood MA., Zeiner SA., Quadros RM., Harms DW., Bonthuis PJ., Gregg C., Ohtsuka M., Gurumurthy CB. and Behlke MA. Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes. Methods. 121-122: 16-28 (2017)

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• Quadros RM.†, Miura H.†, Harms DW., Akatsuka H., Sato T., Aida T., Redder R., Richardson GP., Inagaki Y., Sakai D., Buckley SM., Seshacharyulu P., Batra SK., Behlke MA., Zeiner SA., Jacobi AM., Izu Y., Thoreson WB., Urness LD., Mansour SL.*, Ohtsuka M.* and Gurumurthy CB.* Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins. Genome Biol. 18: 92 (2017) †equal contribution, *correspondence

2016年

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• Inoue M., Ohtsu K., Ohtsuka M., Takafuji K., Harada H., Ishisaki A., Kondo H. "Healing mechanism surrounding transplanted bone using transgenic mice expressing red fluorescent protein in vivo". Journal of Oral Tissue Engineering. 14: 74-82 (2016)

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• Mizutani R., Saiga R., Ohtsuka M., Miura H., Hoshino M., Takeuchi A. and Uesugi K. Three-dimensional X-ray visualization of axonal tracts in mouse brain hemisphere. Sci Rep. 6: 35061 (2016)

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• Kobayashi Y., Kamimura K., Abe H., Yokoo T., Ogawa K., Shinagawa Y., Goto R., Inoue R., Ohtsuka M., Miura H., Kanefuji T., Suda T., Tsuchida M., Aoyagi Y., Zhang G., Liu D. and Terai S. Effects of Fibrotic Tissue on Liver-targeted Hydrodynamic Gene Delivery. Mol Ther Nucleic Acids 5, e359 (2016)

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• Gurumurthy C.B., Quadros R., Sato M., Mashimo T., Lloyd K.C.K. and Ohtsuka M. CRISPR/Cas9 and the Paradigm Shift in Mouse Genome Manipulation Technologies. In: Turksen K (ed) Genome editing. 65-77 (Springer International Publishing) (2016)

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• Gurumurthy C.B., Grati M., Ohtsuka M., Schilit S.L.P., Quadros R. and Liu X.Z. CRISPR: a Versatile Tool for Both Forward and Reverse Genetics Research. Hum. Genet. 135, 971-976 (2016)

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• Schilit S.L.P.*, Ohtsuka M.*, Quadros R. and Gurumurthy C.B. Pronuclear Injection-based Targeted Transgenesis (PITT). Curr. Protoc. Hum. Genet. 91: 15.10.1-15.10.28 (2016) *equal contribution

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• Sato M., Maeda K., Koriyama M., Inada E., Saitoh I., Miura H., Ohtsuka M., Nakamura S., Sakurai T., Watanabe S. and Miyoshi K. The piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs. Int J Mol Sci. 17, E1424 (2016)

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• Tsuchida J., Matsusaka T., Ohtsuka M., Miura H., Okuno Y., Asanuma K., Nakagawa T., Yanagita M. and Mori K. Establishment of Nephrin Reporter Mice and Use for Chemical Screening. PLoS One 11: e0157497 (2016)

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• Motojima M., Tanimoto S., Ohtsuka M., Matsusaka T., Kume T. and Abe K. Characterization of Kidney and Skeleton Phenotypes of Mice Double Heterozygous for Foxc1 and Foxc2. Cells Tissues Organs 201, 380-389 (2016)

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• Sato M., Ohtsuka M., Watanabe S. and Gurumurthy C. B. Nucleic Acids Delivery Methods for Genome Editing in Zygotes and Embryos: the Old, the New, and the Old-New. Biol. Direct 11, 16 (2016)

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• Gurumurthy C.B., Takahashi G., Wada K., Miura H., Sato M. and Ohtsuka M. GONAD: a novel CRISPR/Cas9 genome editing method that does not require ex vivo handling of embryos. Curr. Protoc. Hum. Genet. 88:15.8.1-15.8.12. (2016)

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• Abe H., Kamimura K., Kobayashi Y., Ohtsuka M., Miura H., Ohashi R., Yokoo T., Kanefuji T., Suda T., Tsuchida M., Aoyagi Y., Zhang G., Liu D. and Terai S. Effective Prevention of Liver Fibrosis by Liver-targeted Hydrodynamic Gene Delivery of Matrix Metalloproteinase-13 in a Rat Liver Fibrosis Model. Mol Ther Nucleic Acids 5, e276 (2016)

• Motojima M., Ogiwara S., Matsusaka T., Yong SK., Sagawa N., Abe K. and Ohtsuka M. Conditional knockout of Foxc2 gene in kidney; efficient generation of conditional alleles of single-exon gene by double-selection system. Mamm Genome 27, 62-69 (2016)

2015年

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• Sato T., Akatsuka H., Yamaguchi Y., Miyashita K., Tanaka M., Tamaki T., Ohtsuka M. and Kimura M. Establishment of b-2 microglobulin deficient human iPS cells using CRISPR/Cas9 system. Integr Mol Med 2, 373-377 (2015)

• Miura H., Inoko H., Tanaka M., Nakaoka H., Kimura M., Gurumurthy C. B., Sato M. and Ohtsuka M. Assessment of artificial miRNA architectures for higher knockdown efficiencies without the undesired effects in mice. PLoS ONE 10: e0135919 (2015)

• Miura H., Gurumurthy C. B., Sato T., Sato M. and Ohtsuka M. CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA. Sci Rep. 5: 12799 (2015)

• Sato M., Koriyama M., Watanabe S., Ohtsuka M., Sakurai T., Inada E., Saitoh I., Nakamura S. and Miyoshi K. Direct injection of CRISPR/Cas9-related mRNA into cytoplasm of parthenogenetically activated porcine oocytes causes frequent mosaicism for indel mutations. Int. J. Mol. Sci. 16, 17838-17856 (2015)

• Takahashi G., Gurumurthy C. B., Wada K., Miura H., Sato M. and Ohtsuka M. GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice. Sci Rep. 5: 11406 (2015)

• Sato M., Kagoshima A., Saitoh I., Inada E., Miyoshi K., Ohtsuka M., Nakamura S., Sakurai T. and Watanabe S. Generation of α-1,3-galactosyltransferase-deficient porcine embryonic fibroblasts by CRISPR/Cas9-mediated knock-in of a small mutated sequence and a targeted toxin-based selection system. Reprod. Domest. Anim. 50, 872-880 (2015)

• Inada E., Saitoh I., Watanabe S., Aoki R., Miura H., Ohtsuka M., Murakami T., Sawami T., Yamasaki Y. and Sato M. PiggyBac transposon-mediated gene delivery efficiently generates stable transfectants derived from cultured primary human deciduous tooth dental pulp cells (HDDPCs) and HDDPC-derived iPS cells. Int J Oral Sci. 7, 144-154 (2015)

• Ohtsuka M., Miura H., Mochida K., Hirose M., Hasegawa A., Ogura A., Mizutani R., Kimura M., Isotani A., Ikawa M., Sato M. and Gurumurthy C. B. One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics 16:274 (2015)

• Quadros R.*, Harms D.*, Ohtsuka M.* and Gurumurthy C. B. Insertion of sequences at the original provirus integration site of mouse ROSA26 locus using the CRISPR/Cas9 system. FEBS Open Bio., 5, 191-197 (2015) *equal contribution

• Gurumurthy C. B., Joshi P., Kurz S., Ohtsuka M., Quadros R., Harms D. and Lloyd K.C. Validation of Simple Sequence Length Polymorphism (SSLP) regions of commonly used mouse strains for marker assisted speed congenics screening. Int J Genomics, 2015, 735845 (2015)

• Furukawa S., Kuwajima Y., Chosa N., Satoh K., Ohtsuka M., Miura H., Kimura M., Inoko H., Ishisaki A., Fujimura A. and Miura H. Establishment of immortalized mesenchymal stem cells derived from the submandibular glands of tdTomato transgenic mice. Exp. Ther. Med., 10, 1380-1386 (2015)

• Sato M., Inada E., Saitoh I., Matsumoto Y., Ohtsuka M., Miura H., Nakamura S., Sakurai T. and Watanabe S. A combination of targeted toxin technology and the piggyBac-mediated gene transfer system enables efficient isolation of stable transfectants in nonhuman mammalian cells. Biotechnol. J., 10, 143-153 (2015)

2014年

• Harms DW.*, Quadros RM.*, Seruggia D.*, Ohtsuka M.*, Takahashi G., Montoliu L., and Gurumurthy CB. Mouse Genome Editing Using the CRISPR/Cas System. Curr Protoc Hum Genet. 83:15.7.1-15.7.27. (2014) *equal contribution

• Sato M., Miyoshi K., Nagao Y., Nishi Y., Ohtsuka M., Nakamura S., Sakurai T. and Watanabe S. The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of the α-1,3-galactosyltransferase gene in porcine embryonic fibroblasts. Xenotransplantation, 21, 291-300 (2014)

• Ohtsuka M. Development of pronuclear injection-based targeted transgenesis in mice through Cre–loxP site-specific recombination. in Mouse Genetics: Methods and Protocols (Methods in Molecular Biology). Chapter 1, 3-19 (Humana press) (2014)

• 加賀谷徹、割田貴之、三浦浩美、大塚正人、大久保朋一、坂部貢、木村穣　Neuropathy target esterase（神経障害標的エステラーゼ）遺伝子導入マウスの作製. 臨床環境 23, 34-40. (2014)

• 古川真司、佐藤和朗、藤村朗、大塚正人、三浦廣行　tdTomatoマウス唾液腺における赤色蛍光の局在と唾液腺由来培養細胞の特性. 岩手医科大学歯学雑誌. 39, 14-28. (2014)

• 大塚正人、佐藤正宏：トランスジェニックマウスの基礎と応用．小児外科. 46, 571-574. (2014)

• 大塚正人、角田茂：遺伝子改変法①部位特異的組換え酵素システム．ES iPS細胞実験スタンダード（中辻憲夫 監、末盛博文 編）羊土社 pp.288-299 (2014)

2013年

• Sato M., Inada E., Saitoh I., Ohtsuka M., Nakamura S., Sakurai T. and Watanabe S. Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice. Biotechnol. J., 8, 1355-1361 (2013)

• Mizutani R., Taguchi K., Ohtsuka M., Kimura M., Takeuchi A. Uesugi K. and Suzuki Y. X-ray microtomographic visualization of Escherichia coli by metalloprotein overexpression. J. Synch. Rad., 20, 581-586 (2013)

• Ohtsuka M., Miura H., Hayashi H., Nakaoka H., Kimura M., Sato M., Gurumurthy C. B. and Inoko H. Improvement of pronuclear injection-based targeted transgenesis (PITT) by iCre mRNA-mediated site-specific recombination. Transgenic Res., 22, 873-875 (2013)

• Miura H., Inoko H., Inoue I., Okada Y., Tanaka M., Sato M. and Ohtsuka M. piggyBac-mediated generation of stable transfectants with surface HLA expression from a small number of cells. Anal. Biochem., 437, 29-31 (2013)

• Sato M., Akasaka E., Saitoh I., Ohtsuka M., Nakamura S., Sakurai T. and Watanabe S. Targeted Toxin-Based Selectable Drug-free Enrichment of Mammalian Cells with High Transgene Expression. Biology, 2, 341-355 (2013)

• Sato M., Kubota N, Inada E, Saitoh I., Ohtsuka M., Nakamura S., Sakurai T., Watanabe S. HeLa cells consist of two cell types, as evidenced by cytochemical staining for alkaline phosphatase activity: A possible model for cancer stem cell study. Advances in Stem Cells, 2013, 208514 (2013)

• Ohtsuka M. Development and recent progress in the pronuclear injection-based targeted transgenesis method by site-specific recombination in mice. Proceedings of the Genetic Transformation Technologies (plants and animals). MEDIMOND, 3-6 (2013)

2012年

• Sato M., Ohtsuka M., Nakamura S., Sakurai T., Watanabe S. and Yasuoka Y. Functional recovery of a whole ovary transplanted into syngenic testis in mice. Cloning & Transgenesis, 1, 1000102 (2012)

• Ohtsuka M., Miura H., Gurumurthy C. B., Kimura M., Inoko H., Yoshimura S. and Sato M. Fluorescent transgenic mice suitable for multi-color aggregation chimera studies. Cell Tissue Res. 350, 251-260 (2012)

• Sato M., Akasaka E., Saitoh I., Ohtsuka M., Nakamura S., Sakurai T. and Watanabe S. A simplified protocol for the semi-large scale recovery of plasmids from Escherichia coli grown on agar plates. J Biomed Sci Eng. 5, 406-408 (2012)

• Sato M., Akasaka E., Saitoh I., Ohtsuka M. and Watanabe S. In vivo gene transfer in mouse preimplantation embryos after intraoviductal injection of plasmid DNA and subsequent in vivo electroporation. Syst. Biol. Reprod. Med. 58, 278-287 (2012)

• Sato M., Ohtsuka M., Miura H., Miyoshi K. and Watanabe S. Determination of the optimal concentration of several selective drugs useful for generating multi-transgenic porcine embryonic fibroblasts. Reprod. Domest. Anim. 47, 759-765 (2012)

• Sato M., Akasaka E., Saitoh I., Ohtsuka M. and Watanabe S. Development of a technique for efficient gene transfer to antral follicular cells in the mouse ovary. Syst. Biol. Reprod. Med. 58, 136-141 (2012)

• Ohtsuka M., Miura H., Sato M., Kimura M., Inoko H. and Gurumurthy C. B.  PITT: Pronuclear Injection-based Targeted Transgensis, a Reliable Transgene Expression Method in Mice. Exp. Anim. 61, 489-502. (2012)

• Miura H., Inoko H., Kimura M. and Ohtsuka M. Production of miRNA-based knockdown mice showing reproducible knockdown of the gene of interest. J. Reprod. Develop. 58, A9. (2012)

• Ohtsuka M., Miura H., Nakaoka H. Kimura M., Sato M. and Inoko H. Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs. Transgenic Res. 21, 225-226 (2012)

• 桑島幸紀、大塚正人、石崎明、藤村朗　赤色蛍光強発現遺伝子導入マウスにおける蛍光発現部位の形態学的検討. 岩手医科大学歯学雑誌. 37, 24-37. (2012)

2011年

• Miura H., Inoko H., Inoue I., Tanaka M., Sato M. and Ohtsuka M. Simple cloning strategy using GFPuv gene as positive/negative indicator. Anal. Biochem. 416, 237-239 (2011)

• Yoshii Y., Otomo A., Pan L., Ohtsuka M. and Hadano S. Loss of glial fibrillary acidic protein marginally accelerates disease progression in a SOD1H46R transgenic mouse model of ALS. Neurosci. Res. 70, 321-329 (2011)

• Sato M., Yoshida M., Miyoshi K. Ohtsuka M. and Watanabe S. Cultivation with Untransfected Fibroblasts Stimulates Proliferation of a Single Gene-modified Fibroblast Derived from a Clawn Miniature Swine Fetus. Reprod. Domest. Anim. 46, 911-916 (2011)

• 大塚正人：PITT法——マウス受精卵での部位特異的遺伝子導入法. 実験医学. 29, 2687-2692. (2011)

• Ohtsuka M., Takeda H., and Shimada A. Double anal fin (Da): a medaka mutant exhibiting a mirror-image pattern duplication of the dorsal-ventral axis. in Medaka: Model for Organogesis, Human Diseases and Evolution. Chapter 13, 201-215 (Springer press) (2011)

〜2010年

• Ohtsuka M., Ogiwara S., Miura H., Mizutani A., Warita T., Sato M., Imai K., Hozumi K., Sato T., Tanaka M., Kimura M. and Inoko H. Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression. Nucleic Acids Res. 38, e198 (2010)

• Akasaka E., Watanabe S., Himaki T., Ohtsuka M., Yoshida M., Miyoshi K. and Sato M. Enrichment of xenograft-competent genetically modified pig cells using a targeted toxin, isolectin BS-I-B4 conjugate. Xenotransplantation 17, 81-89 (2010)

• Kokubu C., Horie K., Abe K., Ikeda R., Mizuno S., Uno Y., Ogiwara S., Ohtsuka M., Isotani A., Okabe M., Imai K., and Takeda J. A transposon-based chromosomal engineering method to survey a large cis-regulatory landscape in the mouse genome. Nat. Genet. 41, 946-952 (2009)

• Ohtsuka M., Warita T., Sakurai T., Watanabe S., Inoko H. and Sato M. Development of CRTEIL and CETRIZ, Cre-loxP-based systems, which allow change of expression of red to green or green to red fluorescence upon transfection with a Cre expression vector. J Biomed Biotechnol. 2009, 985140 (2009)

• Ohtsuka, M., Kimura, M., Tanaka, M. and Inoko, H. Recombinant DNA technologies for construction of precisely designed transgene constructs. Curr. Pharm. Biotechnol. 10, 244-251. (2009)

• Ohtsuka M., Inoko H., Kulski J.K. and Yoshimura S. Major histocompatibility complex (Mhc) class Ib gene duplications, organization and expression patterns in mouse strain C57BL/6. BMC Genomics 9, 178 (2008)

• Nakamura S., Watanabe S., Ohtsuka M., Maehara T., Ishihara M., Yokomine T. and Sato M. Cre-loxP System As a Versatile Tool for Conferring Increased Levels of Tissue-Specific Gene Expression from a Weak Promoter. Mol. Reprod. Dev. 75,1085-1093 (2008)

• Ohtsuka M., Kikuchi N., Kimura M., Ozato K. and Yoda K. Genetic mapping of the medaka pectoral-finless (pl) mutant locus. Fish Biol. J. Medaka 11, 15-16 (2007)

• Ohtsuka M., Mizutani A., Kikuti YY., Kulski J.K., Sato M., Kimura M., Tanaka M. and Inoko H. One-step generation of recombineering constructs by asymmetric-end ligation and negative selection. Anal. Biochem. 360, 306-308 (2007)

• Ohtsuka M., Ishii K., Kikuti YY., Warita T., Suzuki D., Sato M., Kimura M. and Inoko H. Construction of Mouse 129/Ola BAC Library for the Targeting Experiments Using E14 Embryonic Stem Cells. Genes Genet. Syst. 81, 143-146 (2006)

• Mizutani A., Ohtsuka M., Kimura M., Tanaka M. and Inoko H. An EYFP insertion mutant containing a modified sequence for potential use as a recombination indicator. Nucleic Acids Symp Ser. 49, 297-298 (2005)

• Sato M., Ohtsuka M. and Ohmi Y. Usefulness of repeated GenomiPhi, a phi29 DNA polymerase-based rolling circle amplification kit, for generation of large amounts of plasmid DNA. Biomol Eng. 22, 129-132. (2005)

• Ohmi Y., Sato M. and Ohtsuka M. Direct subcloning of target region from BAC insert using restriction enzymes that produce non-identical cohesive ends. Nucleic Acids Symp Ser. 48, 141-142. (2004)

• Sato M., Ohtsuka M. and Ohmi Y. Repeated GenomiPhi, phi29 DNA polymerase-based rolling circle amplification, is useful for generating of large amounts of plasmid DNA. Nucleic Acids Symp Ser. 48, 147-148. (2004)

• Ohtsuka M., Horiuchi S., Kulski J.K., Kimura M. and Inoko H. CHOP: visualization of 'wobbling' and isolation of highly conserved regions from aligned DNA sequences. Nucleic Acids Res. 32, W55-58. (2004)

• Ohtsuka M., Kikuchi N., Yokoi H., Kinoshita M., Wakamatsu Y., Ozato K., Takeda H., Inoko H. and Kimura M. Possible roles of zic1 and zic4, identified within the medaka Double anal fin (Da) locus, in dorsoventral patterning of the trunk-tail region (related to phenotypes of the Da mutant). Mech Dev. 121, 873-882. (2004)

• Ohtsuka, M., Kikuchi, N., Ozato, K., Inoko, H. and Kimura, M. Comparative analysis of a 229 kb medaka genomic region, containing the zic1 and zic4 genes, to Fugu, human and mouse. Genomics 83, 1063-1071. (2004)

• Kikuchi, N., Nakamura, S., Ohtsuka M., Kimura, M. and Sato, M. Possible mechanism of gene transfer into early to midgestational mouse fetuses by tail vein injection. Gene Therapy 9, 1529-1541. (2002)

• Ohtsuka, M., Kikuchi, N., Nogami, M., Inoko, H., Ozato, K. and Kimura, M. Rapid screening of a novel arrayed medaka (Oryzias latipes) cosmid library. Mar. Biotechnol. 4, 173-178. (2002)

• 大塚正人：留学記　ドイツ EMBL Heidelberg. Jpn J Clin Ecol (Vol.11 No.1 46-48, 2002)

• Wittbrodt J., Arendt D., Carl M., Loosli F., Martinez J., Quiring R., Bene F. D., Grabher C., Iquel C., Rembold M., Grzebisz E., Krone A., Nowicka A., Wittbrodt B., Poggi L., Ohtsuka M., Schaefer M., Aghaalaei N., Bajoghli B., Gonzalez J. M. R., Arenz A., Tessmar K., Ines M., Baptista M. C. Brain patterning and eye development. EMBL Research report. 93-96 (2002)

• Taniguchi, Y., Suzuki, H., Ohtsuka M., Kikuchi, N., Kimura, M. and Inoko, H. Isolation and characterization of three genes paralogous to mouse . Nucleic Acid Research Supplement No.1, 247-248. (2001)

• 大塚正人、木村穣：メダカ背腹構造突然変異体Daの原因遺伝子のポジショナルクローニング. 蛋白質 核酸 酵素. 45 (増刊), 2864-2871. (2000)

• Ohtsuka, M., Makino, S., Yoda, K., Wada, H., Naruse, K., Mitani, H., Shima, A., Ozato, K., Kimura, M. and Inoko , H. Construction of a linkage map of the medaka (Oryzias latipes) and mapping of the Da mutant locus defective in dorsoventral patterning. Genome Res. 9, 1277-1287. (1999)